The synthesis and biological activities of oligodeoxynucleotides that are covalently linked to psoralen at their 5' ends.
Identifieur interne : 004018 ( Main/Exploration ); précédent : 004017; suivant : 004019The synthesis and biological activities of oligodeoxynucleotides that are covalently linked to psoralen at their 5' ends.
Auteurs : L X Wang [République populaire de Chine] ; D C Yang ; Y Y Lu ; Y. Takagi ; K. Taira ; T. Li ; L H ZhangSource :
- Drug design and discovery [ 1055-9612 ] ; 1995.
Descripteurs français
- KwdFr :
- ARN messager (biosynthèse), Animaux, Cellules cancéreuses en culture, Division cellulaire (), Données de séquences moléculaires, Expression des gènes (), Gènes ras (), Oligonucléotides (), Oligonucléotides (pharmacologie), Oligonucléotides antisens (pharmacologie), Oligonucléotides antisens (synthèse chimique), Protéines proto-oncogènes p21(ras) (biosynthèse), Protéines proto-oncogènes p21(ras) (génétique), Psoralène (), Psoralène (pharmacologie), Souris, Spectrométrie de fluorescence, Spectrophotométrie UV, Séquence d'acides aminés, Séquence nucléotidique, Transfection.
- MESH :
- biosynthèse : ARN messager, Protéines proto-oncogènes p21(ras).
- génétique : Protéines proto-oncogènes p21(ras).
- pharmacologie : Oligonucléotides, Oligonucléotides antisens, Psoralène.
- synthèse chimique : Oligonucléotides antisens.
- Animaux, Cellules cancéreuses en culture, Division cellulaire, Données de séquences moléculaires, Expression des gènes, Gènes ras, Oligonucléotides, Psoralène, Souris, Spectrométrie de fluorescence, Spectrophotométrie UV, Séquence d'acides aminés, Séquence nucléotidique, Transfection.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Cell Division (drug effects), Ficusin (chemistry), Ficusin (pharmacology), Gene Expression (drug effects), Genes, ras (drug effects), Mice, Molecular Sequence Data, Oligonucleotides (chemistry), Oligonucleotides (pharmacology), Oligonucleotides, Antisense (chemical synthesis), Oligonucleotides, Antisense (pharmacology), Proto-Oncogene Proteins p21(ras) (biosynthesis), Proto-Oncogene Proteins p21(ras) (genetics), RNA, Messenger (biosynthesis), Spectrometry, Fluorescence, Spectrophotometry, Ultraviolet, Transfection, Tumor Cells, Cultured.
- MESH :
- chemical , biosynthesis : Proto-Oncogene Proteins p21(ras), RNA, Messenger.
- chemical , chemical synthesis : Oligonucleotides, Antisense.
- chemical , chemistry : Ficusin, Oligonucleotides.
- drug effects : Cell Division, Gene Expression, Genes, ras.
- chemical , genetics : Proto-Oncogene Proteins p21(ras).
- chemical , pharmacology : Ficusin, Oligonucleotides, Oligonucleotides, Antisense.
- Amino Acid Sequence, Animals, Base Sequence, Mice, Molecular Sequence Data, Spectrometry, Fluorescence, Spectrophotometry, Ultraviolet, Transfection, Tumor Cells, Cultured.
Abstract
Four oligodeoxynucleotides (15-mers), designated 13 through 16, and the corresponding conjugates, designated 17 through 20, in which psoralen was covalently linked to the 5' end of each oligonucleotide were synthesized. Compounds 16 and 20 contained a sequence that was complementary to part of the first four codons and the upstream sequence close to the ribosome-binding site of c-Ha-ras mRNA. Compounds 16 and 20 inhibited the growth of cells that had been transformed by the c-Ha-ras plasmid, with accompanying suppression of the expression of the activated c-Ha-ras oncogene. The antisense oligonucleotides 16 and 20 also appeared to cause partial reversion of the major phenotypic characteristics of transformed cells, which included inhibition of anchorage-independent growth. Compound 20, which contained psoralen, was a more efficient inhibitor of the growth of transformed cells than compound 16 without psoralen, a result that suggests psoralen might have increased the ability of the oligodeoxynucleotides to enter the cells.
PubMed: 8872455
Affiliations:
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Le document en format XML
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Takagi</name></author><author><name sortKey="Taira, K" sort="Taira, K" uniqKey="Taira K" first="K" last="Taira">K. Taira</name></author><author><name sortKey="Li, T" sort="Li, T" uniqKey="Li T" first="T" last="Li">T. 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Takagi</name></author><author><name sortKey="Taira, K" sort="Taira, K" uniqKey="Taira K" first="K" last="Taira">K. Taira</name></author><author><name sortKey="Li, T" sort="Li, T" uniqKey="Li T" first="T" last="Li">T. Li</name></author><author><name sortKey="Zhang, L H" sort="Zhang, L H" uniqKey="Zhang L" first="L H" last="Zhang">L H Zhang</name></author></analytic><series><title level="j">Drug design and discovery</title><idno type="ISSN">1055-9612</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cell Division (drug effects)</term><term>Ficusin (chemistry)</term><term>Ficusin (pharmacology)</term><term>Gene Expression (drug effects)</term><term>Genes, ras (drug effects)</term><term>Mice</term><term>Molecular Sequence Data</term><term>Oligonucleotides (chemistry)</term><term>Oligonucleotides (pharmacology)</term><term>Oligonucleotides, Antisense (chemical synthesis)</term><term>Oligonucleotides, Antisense (pharmacology)</term><term>Proto-Oncogene Proteins p21(ras) (biosynthesis)</term><term>Proto-Oncogene Proteins p21(ras) (genetics)</term><term>RNA, Messenger (biosynthesis)</term><term>Spectrometry, Fluorescence</term><term>Spectrophotometry, Ultraviolet</term><term>Transfection</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (biosynthèse)</term><term>Animaux</term><term>Cellules cancéreuses en culture</term><term>Division cellulaire ()</term><term>Données de séquences moléculaires</term><term>Expression des gènes ()</term><term>Gènes ras ()</term><term>Oligonucléotides ()</term><term>Oligonucléotides (pharmacologie)</term><term>Oligonucléotides antisens (pharmacologie)</term><term>Oligonucléotides antisens (synthèse chimique)</term><term>Protéines proto-oncogènes p21(ras) (biosynthèse)</term><term>Protéines proto-oncogènes p21(ras) (génétique)</term><term>Psoralène ()</term><term>Psoralène (pharmacologie)</term><term>Souris</term><term>Spectrométrie de fluorescence</term><term>Spectrophotométrie UV</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Transfection</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Proto-Oncogene Proteins p21(ras)</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ficusin</term><term>Oligonucleotides</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN messager</term><term>Protéines proto-oncogènes p21(ras)</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Division</term><term>Gene Expression</term><term>Genes, ras</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Proto-Oncogene Proteins p21(ras)</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines proto-oncogènes p21(ras)</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Oligonucléotides</term><term>Oligonucléotides antisens</term><term>Psoralène</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Ficusin</term><term>Oligonucleotides</term><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Oligonucléotides antisens</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Mice</term><term>Molecular Sequence Data</term><term>Spectrometry, Fluorescence</term><term>Spectrophotometry, Ultraviolet</term><term>Transfection</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cancéreuses en culture</term><term>Division cellulaire</term><term>Données de séquences moléculaires</term><term>Expression des gènes</term><term>Gènes ras</term><term>Oligonucléotides</term><term>Psoralène</term><term>Souris</term><term>Spectrométrie de fluorescence</term><term>Spectrophotométrie UV</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Four oligodeoxynucleotides (15-mers), designated 13 through 16, and the corresponding conjugates, designated 17 through 20, in which psoralen was covalently linked to the 5' end of each oligonucleotide were synthesized. Compounds 16 and 20 contained a sequence that was complementary to part of the first four codons and the upstream sequence close to the ribosome-binding site of c-Ha-ras mRNA. Compounds 16 and 20 inhibited the growth of cells that had been transformed by the c-Ha-ras plasmid, with accompanying suppression of the expression of the activated c-Ha-ras oncogene. The antisense oligonucleotides 16 and 20 also appeared to cause partial reversion of the major phenotypic characteristics of transformed cells, which included inhibition of anchorage-independent growth. Compound 20, which contained psoralen, was a more efficient inhibitor of the growth of transformed cells than compound 16 without psoralen, a result that suggests psoralen might have increased the ability of the oligodeoxynucleotides to enter the cells.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Li, T" sort="Li, T" uniqKey="Li T" first="T" last="Li">T. Li</name><name sortKey="Lu, Y Y" sort="Lu, Y Y" uniqKey="Lu Y" first="Y Y" last="Lu">Y Y Lu</name><name sortKey="Taira, K" sort="Taira, K" uniqKey="Taira K" first="K" last="Taira">K. Taira</name><name sortKey="Takagi, Y" sort="Takagi, Y" uniqKey="Takagi Y" first="Y" last="Takagi">Y. Takagi</name><name sortKey="Yang, D C" sort="Yang, D C" uniqKey="Yang D" first="D C" last="Yang">D C Yang</name><name sortKey="Zhang, L H" sort="Zhang, L H" uniqKey="Zhang L" first="L H" last="Zhang">L H Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wang, L X" sort="Wang, L X" uniqKey="Wang L" first="L X" last="Wang">L X Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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